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The assay has three components: a kinase-tagged phage, a test compound, and an immobilized ligand that the compound competes with to displace the kinase. The amount of kinase bound to the immobilized ligand is determined using quantitative PCR of the DNA tag. Results for each kinase are reported as \"Percent of control\", where the control is DMSO and where a 100% result means no inhibition of kinase binding to the ligand in the presence of the compound, and where low percent results mean strong inhibition. The KINOMEscan data are presented graphically on TREEspot Kinase Dendrograms (http://www.kinomescan.com/Tools---Resources/Study-Reports---Data-Analysis). For this study, HMS LINCS investigators have graphed results for kinases classified as 35 \"percent of control\" (in the presence of the compound, the kinase is 35% as active for binding ligand in the presence of DMSO), 5 \"percent of control\" and 1 \"percent of control\". 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The assay has three components: a kinase-tagged phage, a test compound, and an immobilized ligand that the compound competes with to displace the kinase. The amount of kinase bound to the immobilized ligand is determined using quantitative PCR of the DNA tag. Results for each kinase are reported as \"Percent of control\", where the control is DMSO and where a 100% result means no inhibition of kinase binding to the ligand in the presence of the compound, and where low percent results mean strong inhibition. The KINOMEscan data are presented graphically on TREEspot Kinase Dendrograms (http://www.kinomescan.com/Tools---Resources/Study-Reports---Data-Analysis). For this study, HMS LINCS investigators have graphed results for kinases classified as 35 \"percent of control\" (in the presence of the compound, the kinase is 35% as active for binding ligand in the presence of DMSO), 5 \"percent of control\" and 1 \"percent of control\". ", "centerurl": "http://lincs.hms.harvard.edu", "description": "For the panel of kinases, the percent of kinase bound by ligand is measured in the presence of GSK461364 at 10 uM of concentration.", "principalinvestigator": "Nathanael Gray", "centerfullname": "HMS LINCS (Harvard Medical School)", "centername": "HMS_LINCS", "ldplink": "http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1017", "path": "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS", "protocol": "LDG-1016_Protocol.pdf", "datemodified": "2015-10-06", "centerletter": "H", "screeninglabinvestigator": "Qingsong Liu", "datasetname": "GSK461364 KINOMEscan", "datasetid": "LDS-1017", "id": "7729", "datereleased": "2011-07-15", "assayname": [ "KINOMEscan kinase-small molecule binding assay" ], "assayformat": "Biochemical", "assaydesignmethod": [ "KINOMEscan" ], "physicaldetection": "Fluorescence intensity", "biologicalprocess": [ "Small molecule metabolic process" ], "technologies": "KINOMEscan", "biologicalbucket": "Binding", 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"RPS6KA4(Kin.Dom.1-N-terminal)", "RPS6KA4(Kin.Dom.2-C-terminal)", "RPS6KA5(Kin.Dom.1-N-terminal)", "RPS6KA5(Kin.Dom.2-C-terminal)", "RSK1(Kin.Dom.1-N-terminal)", "RSK1(Kin.Dom.2-C-terminal)", "RSK2(Kin.Dom.1-N-terminal)", "RSK3(Kin.Dom.1-N-terminal)", "RSK3(Kin.Dom.2-C-terminal)", "RSK4(Kin.Dom.1-N-terminal)", "RSK4(Kin.Dom.2-C-terminal)", "S6K1", "SBK1", "SgK110", "SGK3", "SIK1", "SIK2", "SIK3", "SLK", "SNARK", "SNRK", "SRC", "SRMS", "SRPK1", "SRPK2", "SRPK3", "STK16", "STK33", "STK35", "STK36", "STK39", "SYK", "TAK1", "TAOK1", "TAOK2", "TAOK3", "TBK1", "TEC", "TESK1", "TGFBR1", "TGFBR2", "TIE1", "TIE2", "TLK1", "TLK2", "TNIK", "TNK1", "TNK2", "TNNI3K", "TRKA", "TRKB", "TRKC", "TRPM6", "TSSK1", "TTK", "TXK", "TYK2(JH1domain-catalytic)", "TYK2(JH2domain-pseudokinase)", "TYRO3", "ULK1", "ULK2", "ULK3", "VGFR1", "VGFR2", "VRK2", "WEE1", "WEE2", "YANK1", "YANK2", "YANK3", "YES", "YSK1", "YSK4", "ZAP70" ], "_version_": 1773295666441748480 }, { "centerdatasetid": "http://lincs.hms.harvard.edu/db/datasets/20003/", "funding": "NIH 1U54HG006097-01; NIH 1U54HL127365-01", "projectname": "LINCS phase 1", "assayoverview": "Tang Mitosis/Apoptosis ver.II: Dose response of anti-mitotic compounds in human cancer cell lines at 24, 48 and 72 hours to determine effect on apoptosis, mitosis and cell death. \u003cbr /\u003e\n\u003cbr /\u003e\nIn screening for small-molecule compounds that are effective at killing cancer cells, one-dimensional readout GI50, which is the EC50 value of growth inhibition, is usually used as the only criterion. A major problem with this one-readout approach is that other useful information is discarded, which could be critical for understanding the action of the compounds. In this screen, we use a single-cell-based imaging assay that can report multi-dimensional physiological responses in cells treated with small molecule kinase inhibitors. The data in this dataset are described in PMID: 23788527: Differential Determinants of Cancer Cell Insensitivity to Antimitotic Drugs Discriminated by a One-Step Cell Imaging Assay (J. Biomolecular Screening, 2013). The image analysis algorithm is available at https://github.com/xietiao/Tang_et_al_LINCS_cell_scoring.", "centerurl": "http://lincs.hms.harvard.edu", "description": "-", "principalinvestigator": "Tim Mitchison", "centerfullname": "HMS LINCS (Harvard Medical School)", "centername": "HMS_LINCS", "ldplink": "http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1004", "path": "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS", "protocol": "LDG-1003_Protocol.pdf", "datemodified": "2016-07-12", "centerletter": "H", "publication": "23788527", "screeninglabinvestigator": "Yangzhong Tang", "datasetname": "Tang Mitosis/Apoptosis ver. II", "datasetid": "LDS-1004", "id": "7716", "datereleased": "2011-07-15", "assayname": [ "Fluorescence imaging apoptosis assay", "Fluorescence imaging cell cycle state assay" ], "assayformat": "Cell based", "assaydesignmethod": [ "Fluorescence imaging", "Fluorescence imaging" ], "physicaldetection": "Fluorescence intensity", "biologicalprocess": [ "Apoptotic process", "Cell cycle phase" ], "technologies": "Fluorescence imaging", "biologicalbucket": "Imaging", "endpointcategorization": "Cytological profile", "endpoints": [ "Heatmap" ], "levelspath": [ "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS" ], "datasetlevels": [ "LDS-1004" ], "latestversions": [ "1.1" ], "datalevels": [ "1,2" ], "size": [ "484204" ], "versions": [ "LDS-1004:1.0;1.1" ], "datasetgroup": "LDG-1003", "concentrations": [ "0 uM, 6.3e-05 uM, 0.00019 uM, 0.00056 uM, 0.0017 uM, 0.0051 uM, 0.015 uM, 0.046 uM, 0.137 uM, 0.412 uM, 1.23 uM, 3.7 uM, 11.11 uM" ], "timepoints": [ "24 hr, 48 hr, 72 hr" ], "expentimentalcomments": [ "" ], "statsfields": [ "smallmolecule", "cellline" ], "counts": [ "smallmolecule:8", "cellline:33" ], "statsvalues": [ "8", "33" ], "smlincsidentifier": [ "LSM-1021", "LSM-1041", "LSM-1062", "LSM-1065", "LSM-1066", "LSM-1067", "LSM-1102", "LSM-1105" ], "cellline": [ "5637", "647V", "A375.S2", "AGS", "Ca9-22", "CAL-51", "Calu-1", "Calu-3", "Ca Ski", "COLO 679", "Fu97", "HEC-1", "HLF", "Ishikawa", "Ishikawa (Heraklio) 02 ER-", "JHH-6", "KMRC-20", "KYSE-140", "KYSE-150", "KYSE-450", "LNZTA3WT4", "MDA-MB-435S", "MT-3", "NCI-H1651", "NCI-H1915", "NCI-H2023", "PE/CA-PJ15", "PL4", "SK-OV-3", "SW527", "SW620", "T24", "WiDr" ], "_version_": 1773295666500468736 }, { "centerdatasetid": "http://lincs.hms.harvard.edu/db/datasets/20002/", "funding": "NIH 1U54HG006097-01; NIH 1U54HL127365-01", "projectname": "LINCS phase 1", "assayoverview": "Moerke 3 Color Apoptosis: Dose response of anti-mitotic compounds in human cancer cell lines at 24 and 48 hours to determine their effects on apoptosis and cell death. In this assay, the cell-permeable DNA dye Hoechst 33342 is used to stain the nuclei of all cells. The fluorescent caspase 3 reporter NucView488 stains the nuclei of cells undergoing apoptosis (in which caspase 3 is active), and the cell-impermeable DNA dye TO-PRO3 stains only the nuclei of dead or dying cells in which membrane integrity is compromised.", "centerurl": "http://lincs.hms.harvard.edu", "description": "-", "principalinvestigator": "Tim Mitchison", "centerfullname": "HMS LINCS (Harvard Medical School)", "centername": "HMS_LINCS", "ldplink": "http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1003", "path": "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS", "protocol": "LDG-1002_Protocol.pdf", "datemodified": "2016-07-12", "centerletter": "H", "screeninglabinvestigator": "Nathan Moerke", "datasetname": "Moerke 3 Color Apoptosis", "datasetid": "LDS-1003", "id": "7715", "datereleased": "2011-07-15", "assayname": [ "Fluorescence imaging apoptosis assay" ], "assayformat": "Cell based", "assaydesignmethod": [ "Fluorescence imaging" ], "physicaldetection": "Fluorescence intensity", "biologicalprocess": [ "Apoptotic process" ], "technologies": "Fluorescence imaging", "biologicalbucket": "Imaging", "endpointcategorization": "Cytological profile", "endpoints": [ "Heatmap" ], "levelspath": [ "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS" ], "datasetlevels": [ "LDS-1003" ], "latestversions": [ "1.1" ], "datalevels": [ "1,2" ], "size": [ "49832" ], "versions": [ "LDS-1003:1.0;1.1" ], "datasetgroup": "LDG-1002", "concentrations": [ "0 uM, 0.00006 uM, 0.00019 uM, 0.00057 uM, 0.00170 uM, 0.00508 uM, 0.01524 uM, 0.04573 uM, 0.13718 uM, 0.41152 uM, 1.23457 uM, 3.70371 uM, 11.11111 uM, 33.33333 uM" ], "timepoints": [ "24 hr, 48 hr, 72 hr" ], "expentimentalcomments": [ "" ], "statsfields": [ "smallmolecule", "cellline" ], "counts": [ "smallmolecule:8", "cellline:7" ], "statsvalues": [ "8", "7" ], "smlincsidentifier": [ "LSM-1014", "LSM-1021", "LSM-1041", "LSM-1102", "LSM-1103", "LSM-1105", "LSM-1106", "LSM-42788" ], "cellline": [ "5637", "BPH-1", "HuTu 80", "KYSE-140", "KYSE-180", "NCI-H810", "SK-LMS-1" ], "_version_": 1773295666546606080 }, { "centerdatasetid": "http://lincs.hms.harvard.edu/db/datasets/20001/", "funding": "NIH 1U54HG006097-01; NIH 1U54HL127365-01", "projectname": "LINCS phase 1", "assayoverview": "Moerke 2 Color Apoptosis: IA-LM, IST-MEL1, NCI-H1648, PC-9 and SK-LMS-1 cells. Dose response of anti-mitotic compounds in human cancer cell lines at 24, 48, and 72 hours to determine their effects on apoptosis. In this assay, the cell-permeable DNA dye Hoechst 33342 is used to stain the nuclei of all cells. The fluorescent caspase 3 reporter NucView488 stains the nuclei of cells undergoing apoptosis (in which caspase 3 is active).", "centerurl": "http://lincs.hms.harvard.edu", "description": "-", "principalinvestigator": "Tim Mitchison", "centerfullname": "HMS LINCS (Harvard Medical School)", "centername": "HMS_LINCS", "ldplink": "http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1002", "path": "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS", "protocol": "LDG-1001_Protocol.pdf", "datemodified": "2016-07-12", "centerletter": "H", "screeninglabinvestigator": "Nathan Moerke", "datasetname": "Moerke 2 Color Apoptosis", "datasetid": "LDS-1002", "id": "7714", "datereleased": "2011-07-15", "assayname": [ "Fluorescence imaging apoptosis assay" ], "assayformat": "Cell based", "assaydesignmethod": [ "Fluorescence imaging" ], "physicaldetection": "Fluorescence intensity", "biologicalprocess": [ "Apoptotic process" ], "technologies": "Fluorescence imaging", "biologicalbucket": "Imaging", "endpointcategorization": "Cytological profile", "endpoints": [ "Heatmap" ], "levelspath": [ "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS" ], "datasetlevels": [ "LDS-1002" ], "latestversions": [ "1.1" ], "datalevels": [ "1,2" ], "size": [ "40674" ], "versions": [ "LDS-1002:1.0;1.1" ], "datasetgroup": "LDG-1001", "concentrations": [ "0 uM, 0.00006 uM, 0.00019 uM, 0.00057 uM, 0.00170 uM, 0.00508 uM, 0.01524 uM, 0.04573 uM, 0.13718 uM, 0.41152 uM, 1.23457 uM, 3.70371 uM, 11.11111 uM, 33.33333 uM" ], "timepoints": [ "24 hr, 48 hr, 72 hr" ], "expentimentalcomments": [ "" ], "statsfields": [ "smallmolecule", "cellline" ], "counts": [ "smallmolecule:8", "cellline:5" ], "statsvalues": [ "8", "5" ], "smlincsidentifier": [ "LSM-1014", "LSM-1021", "LSM-1041", "LSM-1102", "LSM-1103", "LSM-1105", "LSM-1106", "LSM-42788" ], "cellline": [ "COLO 800", "IA-LM", "IST-MEL1", "NCI-H1648", "PC-9" ], "_version_": 1773295666620006400 }, { "centerdatasetid": "http://lincs.hms.harvard.edu/db/datasets/20023/", "funding": "1U54HG006097-01", "projectname": "LINCS phase 1", "assayoverview": "The KINOMEscan assay platform is based on a competition binding assay that is run for a compound of interest against each of a panel of 317 to 456 kinases. The assay has three components: a kinase-tagged phage, a test compound, and an immobilized ligand that the compound competes with to displace the kinase. The amount of kinase bound to the immobilized ligand is determined using quantitative PCR of the DNA tag. Results for each kinase are reported as \"Percent of control\", where the control is DMSO and where a 100% result means no inhibition of kinase binding to the ligand in the presence of the compound, and where low percent results mean strong inhibition. The KINOMEscan data are presented graphically on TREEspot Kinase Dendrograms (http://www.kinomescan.com/Tools---Resources/Study-Reports---Data-Analysis). For this study, HMS LINCS investigators have graphed results for kinases classified as 35 \"percent of control\" (in the presence of the compound, the kinase is 35% as active for binding ligand in the presence of DMSO), 5 \"percent of control\" and 1 \"percent of control\". ", "centerurl": "http://lincs.hms.harvard.edu", "description": "For the panel of kinases, the percent of kinase bound by ligand is measured in the presence of SB590885 at 10 uM of concentration.", "principalinvestigator": "Nathanael Gray", "centerfullname": "HMS LINCS (Harvard Medical School)", "centername": "HMS_LINCS", "ldplink": "http://lincsportal.ccs.miami.edu/datasets/#/view/LDS-1009", "path": "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS", "protocol": "LDG-1008_Protocol.pdf", "datemodified": "2015-10-06", "centerletter": "H", "screeninglabinvestigator": "Qingsong Liu", "datasetname": "SB590885 KINOMEscan", "datasetid": "LDS-1009", "id": "7721", "datereleased": "2011-07-15", "assayname": [ "KINOMEscan kinase-small molecule binding assay" ], "assayformat": "Biochemical", "assaydesignmethod": [ "KINOMEscan" ], "physicaldetection": "Fluorescence intensity", "biologicalprocess": [ "Small molecule metabolic process" ], "technologies": "KINOMEscan", "biologicalbucket": "Binding", "endpointcategorization": "Protein binding profile", "endpoints": [ "percentControl" ], "levelspath": [ "/projects/ccs/bd2klincs/LINCS_Data/HMS_LINCS" ], "datasetlevels": [ "LDS-1009" ], "latestversions": [ "1.1" ], "datalevels": [ "2" ], "size": [ "12895" ], "versions": [ "LDS-1009:1.0;1.1" ], "datasetgroup": "LDG-1008", "concentrations": [ "10 uM" ], "timepoints": [ "" ], "expentimentalcomments": [ "" ], "toollink": [ "http://amp.pharm.mssm.edu/Harmonizome/dataset/LINCS+KinomeScan+Kinase+Inhibitor+Targets", "http://life.ccs.miami.edu/life/search?load\u003dAssayTypeName\u0026search\u003dconcept%3A%26quot%3BKINOMEscan+assay%26quot%3B\u0026q\u003dconcept%3A%26quot%3BKINOMEscan+assay%26quot%3B" ], "tool": [ "Harmonizome", "Life" ], "statsfields": [ "protein", "smallmolecule" ], "counts": [ "protein:442", "smallmolecule:1" ], "statsvalues": [ "442", "1" ], "smlincsidentifier": [ "LSM-42746" ], "protein": [ "AAK1", "ABL1(E255K)-phosphorylated", "ABL1(F317I)-nonphosphorylated", "ABL1(F317I)-phosphorylated", "ABL1(F317L)-nonphosphorylated", "ABL1(F317L)-phosphorylated", "ABL1(H396P)-nonphosphorylated", "ABL1(H396P)-phosphorylated", "ABL1(M351T)-phosphorylated", "ABL1-nonphosphorylated", "ABL1-phosphorylated", "ABL1(Q252H)-nonphosphorylated", "ABL1(Q252H)-phosphorylated", "ABL1(T315I)-nonphosphorylated", "ABL1(T315I)-phosphorylated", "ABL1(Y253F)-phosphorylated", "ABL2", "ACVR1", "ACVR1B", "ACVR2A", "ACVR2B", "ACVRL1", "ADCK3", "ADCK4", "AKT1", "AKT2", "AKT3", "ALK", "AMPK-alpha1", "AMPK-alpha2", "ANKK1", "AURKA", "AURKB", "AURKC", "AXL", "BIKE", "BLK", "BMPR1A", "BMPR1B", "BMPR2", "BMX", "BRAF", "BRAF(V600E)", "BRSK1", "BRSK2", "BTK", "CAMK1", "CAMK1D", "CAMK1G", "CAMK2A", "CAMK2B", "CAMK2G", "CAMKK2", "CASK", "CDC2L1", "CDC2L2", "CDC2L5", "CDK11", "CDK14", "CDK15", "CDK16", "CDK2", "CDK3", "CDK4-cyclinD1", "CDK4-cyclinD3", "CDK5", "CDK7", "CDK8", "CDK9", "CDKL1", "CDKL2", "CDKL3", "CDKL5", "CHEK1", "CHK2", "CIT", "CLK1", "CLK2", "CLK3", "CLK4", "CSF1R", 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"MP2K7", "MRCKA", "MRCKB", "MST1", "MST1R", "MST2", "MST3", "MST4", "MTOR", "MUSK", "MYLK", "MYLK2", "MYLK4", "MYO3A", "MYO3B", "NDR1", "NDR2", "NEK1", "NEK11", "NEK2", "NEK3", "NEK4", "NEK5", "NEK6", "NEK7", "NEK9", "NIM1", "NLK", "NUAK1", "OSR1", "PAK1", "PAK2", "PAK3", "PAK4", "PAK6", "PAK7", "PCTK2", "PCTK3", "PDPK1", "PFCDPK1(P.falciparum)", "PFPK5(P.falciparum)", "PGFRA", "PGFRB", "PHKG1", "PHKG2", "PI42C", "PIK3C2B", "PIK3C2G", "PIK3CA", "PIK3CA(C420R)", "PIK3CA(E542K)", "PIK3CA(E545A)", "PIK3CA(E545K)", "PIK3CA(H1047L)", "PIK3CA(H1047Y)", "PIK3CA(I800L)", "PIK3CA(M1043I)", "PIK3CA(Q546K)", "PIK3CB", "PIK3CD", "PIK3CG", "PIK4CB", "PIM1", "PIM2", "PIM3", "PIP5K1A", "PIP5K1C", "PIP5K2B", "PKAC-alpha", "PKAC-beta", "PKMYT1", "PKN1", "PKN2", "PKNB(M.tuberculosis)", "PLK1", "PLK2", "PLK3", "PLK4", "PRKR", "PRKX", "PRP4", "PTK6", "RAF1", "RET", "RET(M918T)", "RET(V804L)", "RET(V804M)", "RIOK1", "RIOK2", "RIOK3", "RIPK1", "RIPK2", "RIPK4", "RIPK5", "ROCK1", "ROCK2", "ROS1", "RPS6KA4(Kin.Dom.1-N-terminal)", "RPS6KA4(Kin.Dom.2-C-terminal)", "RPS6KA5(Kin.Dom.1-N-terminal)", "RPS6KA5(Kin.Dom.2-C-terminal)", "RSK1(Kin.Dom.1-N-terminal)", "RSK1(Kin.Dom.2-C-terminal)", "RSK2(Kin.Dom.1-N-terminal)", "RSK3(Kin.Dom.1-N-terminal)", "RSK3(Kin.Dom.2-C-terminal)", "RSK4(Kin.Dom.1-N-terminal)", "RSK4(Kin.Dom.2-C-terminal)", "S6K1", "SBK1", "SgK110", "SGK3", "SIK1", "SIK2", "SIK3", "SLK", "SNARK", "SNRK", "SRC", "SRMS", "SRPK1", "SRPK2", "SRPK3", "STK16", "STK33", "STK35", "STK36", "STK39", "SYK", "TAK1", "TAOK1", "TAOK2", "TAOK3", "TBK1", "TEC", "TESK1", "TGFBR1", "TGFBR2", "TIE1", "TIE2", "TLK1", "TLK2", "TNIK", "TNK1", "TNK2", "TNNI3K", "TRKA", "TRKB", "TRKC", "TRPM6", "TSSK1", "TTK", "TXK", "TYK2(JH1domain-catalytic)", "TYK2(JH2domain-pseudokinase)", "TYRO3", "ULK1", "ULK2", "ULK3", "VGFR1", "VGFR2", "VRK2", "WEE1", "WEE2", "YANK1", "YANK2", "YANK3", "YES", "YSK1", "YSK4", "ZAP70" ], "_version_": 1773295666667192320 } ]}}