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Dataset Information
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Dataset Name:
GCP aggregated data - CRISPR perturbations

Dataset Description:


--Data Files in Package:
GCP-CRISPR-Level4.2017_10_18.gct

--Metadata Files In Package:
Cell_Line_Metadata.txt
Nucleic_Acid_Metadata.txt
Peptide_Probe_Metadata.txt

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Center-specific Information
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Center-specific Name:
PCCSE_LINCS

Center-specific Dataset ID:
LDS-1498

Center-specific Dataset Link:
https://panoramaweb.org/labkey/project/LINCS/GCP/begin.view?

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Assay Information
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Assay Overview:
Global chromatin profiling technology is a mass spectrometry-based assay to identify and quantify post-translational modifications on histone proteins from bulk chromatin. The assay quantifies around 60 different combinations of histone modifications on H3, with even more possible combinations on H4, H2A (including isoforms), and H2B. GCP can be considered as orthogonal to ChIP-Seq. Instead of characterizing one histone mark at a time with locus resolution across the genome, one can profile almost all combinations of histone marks together in bulk chromatin with no genomic resolution. The technique is particularly useful in assessing the functional consequences of genetic mutations to the epigenetic machinery and can be employed to make connections among biological conditions and perturbations.

Date Updated:

Date Retrieved from Center:
Fri Oct 20 00:00:00 2017

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Metadata Information
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Metadata information regarding the entities used in the experiments is included in the accompanied metadata. A metadata file per entity category is included in the package. For example, the metadata for all the cell lines that were used in the dataset are included in the Cell_Lines_Metadata.txt file.
Descriptions for each metadata field can be found here: http://www.lincsproject.org/data/data-standards/
