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Dataset Information
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Dataset Name:
hmec122l_ss1

Dataset Description:
HMEC122L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

--Data Files in Package:
LI8X00641_Level1.tsv
LI8X00642_Level1.tsv
LI8X00643_Level1.tsv
LI8X00644_Level1.tsv
LI8X00645_Level1.tsv
LI8X00646_Level1.tsv
LI8X00647_Level1.tsv
LI8X00648_Level1.tsv
MEMA HMEC122l_SS1 Experimental Metadata.xlsx

--Metadata Files In Package:
Protein_Metadata.txt
Cell_Line_Metadata.txt
Unclassified_Perturbabens.txt

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Center-specific Information
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Center-specific Name:
MEP_LINCS

Center-specific Dataset ID:
LDS-1427

Center-specific Dataset Link:
https://www.synapse.org/#!Synapse:syn2862345/wiki/394513

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Assay Information
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Assay Protocol:
https://www.synapse.org/#!Synapse:syn2862345/wiki/231722

Date Updated:

Date Retrieved from Center:
Fri Oct 13 00:00:00 2017
Fri Oct 13 00:00:00 2017

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Metadata Information
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Metadata information regarding the entities used in the experiments is included in the accompanied metadata. A metadata file per entity category is included in the package. For example, the metadata for all the cell lines that were used in the dataset are included in the Cell_Lines_Metadata.txt file.
Descriptions for each metadata field can be found here: http://www.lincsproject.org/data/data-standards/
