centerdatasetid	cl_center_batch_id	cl_lincs_id	cl_name	cl_alternative_names	cl_alternative_ids	cl_center_canonical_id	cl_relevant_citations	cl_center_name	cl_provider_name	cl_provider_catalog_id	cl_provider_batch_id	cl_organism	cl_organ	cl_tissue	cl_cell_type	cl_cell_type_detail	cl_donor_sex	cl_donor_age	cl_donor_ethnicity	cl_donor_health_status	cl_disease	cl_disease_detail	cl_known_mutations	cl_mutation_citations	cl_molecular_features	cl_genetic_modification	cl_growth_properties	cl_recomended_culture_conditions	cl_related_projects	cl_verification_reference_profile	cl_reference_source	cl_cell_markers	cl_gonosome_code	cl_disease_site_onset	cl_disease_age_onset	cl_donor_age_death	cl_donor_disease_duration	cl_precursor_cell_name	cl_precursor_cell_lincs_id	cl_precursor_cell_center_batch_id	cl_production_details	cl_quality_verification	cl_transient_modification	cl_passage_number	cl_source_information	cl_date_received	cl_center_specific_code
20269	50029-1	LCL-1460	MCF-7	ssMCF-7, MCF 7, Michigan Cancer Foundation-7, MCF7/WT, MCF7-CTRL, IBMF-7, ssMCF7, MCF.7, MCF7	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007606""  target=""blank"">CLO_0007606</a>"	50029		HMS_LINCS	ATCC	HTB-22		Homo sapiens	breast		epithelial		female	0	Caucasian	disease	breast adenocarcinoma	DOID:3458		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905946""  target=""blank"">COSS905946</a>"		none	adherent	From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%. Protocol: Remove culture medium to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended		(1.)DNA Profile (STR, source: ATCC): Amelogenin: X  CSF1PO: 10  D13S317: 11  D16S539: 11,12  D5S818: 11,12  D7S820: 8,9  THO1: 6  TPOX: 9,12  vWA: 14,15 (2.)STR_Amelogenin_X, STR_CSF1PO_10, STR_D13S317_11, STR_D16S539_11_12, STR_D5S818_11_12, STR_D7S820_8_9, STR_THO1_6, STR_TPOX_9_12, STR_vWA_14_15	"<a href = ""http://www.atcc.org/Products/All/HTB-22"" target=""blank"">ATCC HTB-22</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC.		
20269	50057-1	LCL-1475	SK-BR-3	Sk-Br-3, SKBR3, SkBr3, SK-Br-3, SKBr3, SKBr-3, SK-BR3, SKBR-3	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009034""  target=""blank"">CLO_0009034</a>"	50057		HMS_LINCS	ATCC	HTB-30		Homo sapiens	breast	breast	epithelial		female	0	Caucasian		adenocarcinoma, breast carcinoma	DOID:3459				none	adherent	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5%  Temperature: 37.0 deg C  Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended  Medium Renewal: 2 to 3 times per week		DNA Profile (STR, source: ATCC):  Amelogenin: X  CSF1PO: 12  D13S317: 11,12  D16S539: 9  D5S818: 9,12  D7S820: 9,12  THO1: 8,9  TPOX: 8,11  vWA: 17	"<a href = ""http://www.atcc.org/Products/All/HTB-30"" target=""blank"">ATCC HTB-30</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50058-1	LCL-1461	MDA-MB-231	MDA Mb231, MDA MB231, MDA-MB 231, MDA.MB.231, MDA MB 231, MB231, MDA231, MDA-231, MDAMB231, MDA-MB231	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007634""  target=""blank"">CLO_0007634</a>"	50058		HMS_LINCS	ATCC	HTB-26		Homo sapiens	breast	breast	epithelial		female	0	Caucasian		breast adenocarcinoma	DOID:3458		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905960""  target=""blank"">COSS905960</a>"		none	adherent	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100%  Temperature: 37.0 deg C  Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended  Medium Renewal: 2 to 3 times per week		DNA Profile (STR, source: ATCC):  Amelogenin: X  CSF1PO: 12,13  D13S317: 13  D16S539: 12  D5S818: 12  D7S820: 8,9  THO1: 7,9.3  TPOX: 8,9  vWA: 15,18	"<a href = ""http://www.atcc.org/Products/All/HTB-26"" target=""blank"">ATCC HTB-26</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50091-1	LCL-1462	AU565	AU 565, AU-565	"<a href = ""http://purl.obolibrary.org/obo/CLO_0001769""  target=""blank"">CLO_0001769</a>"	50091		HMS_LINCS	ATCC	CRL-2351		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=910704""  target=""blank"">COSS910704</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2351"" target=""blank"">ATCC CRL-2351</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50105-1	LCL-1476	BT-20	BT20, BT 20	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002041""  target=""blank"">CLO_0002041</a>"	50105		HMS_LINCS	ATCC	HTB-19		Homo sapiens	breast	breast			female	0	Caucasian		ductal carcinoma, breast carcinoma	DOID:3459		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=906801""  target=""blank"">COSS906801</a>"		none	adherent	From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-19"" target=""blank"">ATCC HTB-19</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50106-1	LCL-1308	BT-474	Bt-474, BT474	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002042""  target=""blank"">CLO_0002042</a>"	50106		HMS_LINCS	ATCC	HTB-20		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=946359""  target=""blank"">COSS946359</a>"		none	adherent	From MGH/CMT as specified by cell provider: Hybri-Care Medium ATCC 46-X, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-20"" target=""blank"">ATCC HTB-20</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50107-1	LCL-1309	BT-483	BT483	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002043""  target=""blank"">CLO_0002043</a>"	50107		HMS_LINCS	ATCC	HTB-121		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=949093""  target=""blank"">COSS949093</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%. Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-121"" target=""blank"">ATCC HTB-121</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50108-1	LCL-1310	BT-549	BT 549, BT.549, BT549	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002044""  target=""blank"">CLO_0002044</a>"	50108		HMS_LINCS	ATCC	HTB-122		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905951""  target=""blank"">COSS905951</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.023 IU/ml insulin and 10% fetal bovine serum. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-122"" target=""blank"">ATCC HTB-122</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50131-1	LCL-1466	CAMA-1	CAMA, Cama-1, CAMA1	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002194""  target=""blank"">CLO_0002194</a>"	50131		HMS_LINCS	ATCC	HTB-21		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=946382""  target=""blank"">COSS946382</a>"		none	adherent	From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-21"" target=""blank"">ATCC HTB-21</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50205-1	LCL-1329	HCC1143	HCC-1143	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003630""  target=""blank"">CLO_0003630</a>"	50205		HMS_LINCS	ATCC	CRL-2321		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749710""  target=""blank"">COSS749710</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2321"" target=""blank"">ATCC CRL-2321</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50206-1	LCL-1330	HCC1395	HCC-1395, SCC-1395	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003634""  target=""blank"">CLO_0003634</a>"	50206		HMS_LINCS	ATCC	CRL-2324		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749712""  target=""blank"">COSS749712</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2324"" target=""blank"">ATCC CRL-2324</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50207-1	LCL-1314	HCC1419	HCC-1419	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003636""  target=""blank"">CLO_0003636</a>"	50207		HMS_LINCS	ATCC	CRL-2326		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=907045""  target=""blank"">COSS907045</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Growth Conditions: The line grows as large epithelial attached cells in island-like formation.  Subculturing: Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2326"" target=""blank"">ATCC CRL-2326</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50208-1	LCL-1467	HCC1428	HCC-1428	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003637""  target=""blank"">CLO_0003637</a>"	50208		HMS_LINCS	ATCC	CRL-2327		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=1290905""  target=""blank"">COSS1290905</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2327"" target=""blank"">ATCC CRL-2327</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50210-1	LCL-1480	HCC1569	HCC-1569	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003640""  target=""blank"">CLO_0003640</a>"	50210		HMS_LINCS	ATCC	CRL-2330		Homo sapiens	breast				female	0			DOID:3459, breast carcinoma	breast carcinoma; metastatic carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=907046""  target=""blank"">COSS907046</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2330"" target=""blank"">ATCC CRL-2330</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50211-1	LCL-1960	HCC1806	HCC-1806, Hcc1806	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003644""  target=""blank"">CLO_0003644</a>"	50211		HMS_LINCS	ATCC	CRL-2335		Homo sapiens	breast				female	0			DOID:7459, primary acantholytic squamous cell carcino	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=907047""  target=""blank"">COSS907047</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2335"" target=""blank"">ATCC CRL-2335</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50212-1	LCL-1331	HCC1937	HCC-1937	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003645""  target=""blank"">CLO_0003645</a>"	50212		HMS_LINCS	ATCC	CRL-2336		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749714""  target=""blank"">COSS749714</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2336"" target=""blank"">ATCC CRL-2336</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50213-1	LCL-1332	HCC1954	HCC-1954	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003647""  target=""blank"">CLO_0003647</a>"	50213		HMS_LINCS	ATCC	CRL-2338		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749709""  target=""blank"">COSS749709</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2338"" target=""blank"">ATCC CRL-2338</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50214-1	LCL-1333	HCC202	HCC-202, HCC0202	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003649""  target=""blank"">CLO_0003649</a>"	50214		HMS_LINCS	ATCC	CRL-2316		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=1290906""  target=""blank"">COSS1290906</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2316"" target=""blank"">ATCC CRL-2316</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50216-1	LCL-1334	HCC38	HCC0038, Hcc38, HCC-38, HCC 38	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003655""  target=""blank"">CLO_0003655</a>"	50216		HMS_LINCS	ATCC	CRL-2314		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749717""  target=""blank"">COSS749717</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2314"" target=""blank"">ATCC CRL-2314</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50219-1	LCL-1335	HCC70	HCC0070, HCC 70, HCC-70	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003658""  target=""blank"">CLO_0003658</a>"	50219		HMS_LINCS	ATCC	CRL-2315		Homo sapiens	breast				female	0			DOID:3008, primary ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=907048""  target=""blank"">COSS907048</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-2315"" target=""blank"">ATCC CRL-2315</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50238-1	LCL-1315	Hs 578T	Hs-578-T, HS 578T, Hs-578T, HS-578T, Hs_578t, HS-578-T, Hs 578.T, HS578T, Hs578T, Hs578t, HS0578T, 578T, HS578, Hs578, Homo sapiens No. 578, tumor cells	"<a href = ""http://purl.obolibrary.org/obo/CLO_0004009""  target=""blank"">CLO_0004009</a>"	50238		HMS_LINCS	ATCC	HTB-126		Homo sapiens	breast	breast			female	0	Caucasian		breast ductal carcinoma	DOID:3008		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905957""  target=""blank"">COSS905957</a>"		none	adherent	From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mg/ml bovine insulin and 10% fetal bovine serum. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-126"" target=""blank"">ATCC HTB-126</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50324-1	LCL-1484	MDA-MB-157	MDA-MB157, MB157, MB 157, MDA157, MDA-157, MDAMB157	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007544""  target=""blank"">CLO_0007544</a>"	50324		HMS_LINCS	ATCC	CRL-7721		Homo sapiens	breast				female	0			DOID:3459, breast carcinoma					none	adherent	From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: 1:2 to 1:3			"<a href = ""http://www.atcc.org/Products/All/CRL-7721"" target=""blank"">ATCC CRL-7721</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50327-1	LCL-1316	MDA-MB-134-VI	MDA134, MM134, MDA-134, MDAMB134, MDA-MB-134, MDA-MB-134VI, MDA-MB-134 VI, MDAMB134VI	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007631""  target=""blank"">CLO_0007631</a>"	50327		HMS_LINCS	ATCC	HTB-23		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma				none	lightly adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-23"" target=""blank"">ATCC HTB-23</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50328-1	LCL-1916	MDA-MB-157	MDA-157, MDAMB157, MB157, MDA157, MDA-MB157, MB 157	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007632""  target=""blank"">CLO_0007632</a>"	50328		HMS_LINCS	ATCC	HTB-24		Homo sapiens	breast				female	0			DOID:5605, breast medullary carcinoma			"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=925338""  target=""blank"">COSS925338</a>"		none	adherent	From MGH/CMT as specified by cell provider: LLeibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C without CO2. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-24"" target=""blank"">ATCC HTB-24</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50329-1	LCL-1317	MDA-MB-175-VII	MDAMB175VII, MDA MB 175 VII, MDA-MB-175VII, MDA-MB-175, MDAMB175, MDA-175, MDA175	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007633""  target=""blank"">CLO_0007633</a>"	50329		HMS_LINCS	ATCC	HTB-25		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=908120""  target=""blank"">COSS908120</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol:  Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-25"" target=""blank"">ATCC HTB-25</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50331-1	LCL-1468	MDA-MB-361	MDA-MB361, MDA MB 361, MDA361, MDA-361, MDAMB361, MDA-MB 361	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007636""  target=""blank"">CLO_0007636</a>"	50331		HMS_LINCS	ATCC	HTB-27		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=908121""  target=""blank"">COSS908121</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-27"" target=""blank"">ATCC HTB-27</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50332-2	LCL-1469	MDA-MB-415	MDAMB415, MDA-MB415, MDA415, MDA-415	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007637""  target=""blank"">CLO_0007637</a>"	50332		HMS_LINCS	ATCC	HTB-128		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=924240""  target=""blank"">COSS924240</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%. Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-128"" target=""blank"">ATCC HTB-128</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50333-1	LCL-1470	MDA-MB-436	MDAMB436, MDA-436, MDA MB 436, MDA-Mb-436, MDA-MB436, MDA436	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007639""  target=""blank"">CLO_0007639</a>"	50333		HMS_LINCS	ATCC	HTB-130		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=1240172""  target=""blank"">COSS1240172</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%. Subculturing: Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-130"" target=""blank"">ATCC HTB-130</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50334-1	LCL-1485	MDA-MB-453	MDA-453, MDAMB453, MDA-MB453, MDA MB 453, MDA-MB 453, MDA453	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007640""  target=""blank"">CLO_0007640</a>"	50334		HMS_LINCS	ATCC	HTB-131		Homo sapiens	breast				female	0			DOID:3459, breast carcinoma	breast carcinoma; metastatic carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=908122""  target=""blank"">COSS908122</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C without CO2. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-131"" target=""blank"">ATCC HTB-131</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50335-1	LCL-1471	MDA-MB-468	MB468, MDA-468, MDA-MB 468, MDA-MB468, MDAMB468, MDA468	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007641""  target=""blank"">CLO_0007641</a>"	50335		HMS_LINCS	ATCC	HTB-132		Homo sapiens	breast				female	0			DOID:3458, adenocarcinoma	breast adenocarcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=908123""  target=""blank"">COSS908123</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-132"" target=""blank"">ATCC HTB-132</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50541-1	LCL-1486	T-47D	T47D, T47D:A, T47-D, T-47-D	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009251""  target=""blank"">CLO_0009251</a>"	50541		HMS_LINCS	European Collection of Cell Cultures (ECACC)	85102201		Homo sapiens	breast				female	0			DOID:3459, breast carcinoma			"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905945""  target=""blank"">COSS905945</a>"		none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37C.			"<a href = ""http://www.atcc.org/Products/All/HTB-133"" target=""blank"">ATCC HTB-133</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ECACC		
20269	50556-1	LCL-1319	UACC-812	UACC 812, UACC812	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009467""  target=""blank"">CLO_0009467</a>"	50556		HMS_LINCS	ATCC	CRL-1897		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=910910""  target=""blank"">COSS910910</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum. Growth Conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium. Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-1897"" target=""blank"">ATCC CRL-1897</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50557-1	LCL-1320	UACC-893	UACC 893, UACC893	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009468""  target=""blank"">CLO_0009468</a>"	50557		HMS_LINCS	ATCC	CRL-1902		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=909778""  target=""blank"">COSS909778</a>"		none	adherent	From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-1902"" target=""blank"">ATCC CRL-1902</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50574-1	LCL-1321	ZR-75-1	ZR751, ZR75-1, ZR75_1	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009727""  target=""blank"">CLO_0009727</a>"	50574		HMS_LINCS	ATCC	CRL-1500		Homo sapiens	breast				female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma				none	adherent	From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended			"<a href = ""http://www.atcc.org/Products/All/CRL-1500"" target=""blank"">ATCC CRL-1500</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50576-1	LCL-2081	184B5	H184B5, B5/589, 184-B5	"<a href = ""http://purl.obolibrary.org/obo/CLO_0001138""  target=""blank"">CLO_0001138</a>"	50576		HMS_LINCS	ATCC	CRL-8799		Homo sapiens	breast		epithelial		female	0			not diseased					none	adherent				"<a href = ""http://www.atcc.org/Products/All/CRL-8799"" target=""blank"">ATCC CRL-8799</a>"										Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established. The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty.				Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50578-1	LCL-1324	HCC1187	HCC-1187	"<a href = ""http://purl.obolibrary.org/obo/CLO_0003632""  target=""blank"">CLO_0003632</a>"	50578		HMS_LINCS	ATCC	CRL-2322		Homo sapiens	breast	duct	epithelial		female	0			DOID:3008, ductal carcinoma	breast ductal carcinoma		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=749711""  target=""blank"">COSS749711</a>"		none	mixed, adherent and suspension				"<a href = ""http://www.atcc.org/Products/All/CRL-2322"" target=""blank"">ATCC CRL-2322</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50583-1	LCL-2085	MCF-10A	Michigan Cancer Foundation-10A, MCF10a, MCF10A, MCF.10A, MCF 10A	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007599""  target=""blank"">CLO_0007599</a>"	50583		HMS_LINCS	ATCC	CRL-10317		Homo sapiens	breast	breast, mammary_gland_connective_tissue	epithelial		female	0	Caucasian	disease	breast fibrocystic disease, fibrocystic disease	non-tumorigenic				none	adherent			Isoenzymes#_AK1#_1, Isoenzymes#_ESD#_1, Isoenzymes#_G6PD#_B, Isoenzymes#_GLO1#_1#_2, Isoenzymes#_PGM1#_1#_2, Isoenzymes#_PGM3#_1, STR#_Amelogenin#_X, STR#_CSF1PO#_10#_12, STR#_D13S317#_8#_9, STR#_D16S539#_11#_12, STR#_D5S818#_10#_13, STR#_D7S820#_10#_11, STR#_THO1#_8#_93, STR#_TPOX#_9#_11, STR#_vWA#_15#_17	"<a href = ""http://www.atcc.org/Products/All/CRL-10317"" target=""blank"">ATCC CRL-10317</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	50584-1	LCL-2102	MCF-10F	MCF 10F, MCF10F, Michigan Cancer Foundation-10F	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007600""  target=""blank"">CLO_0007600</a>"	50584		HMS_LINCS	ATCC	CRL-10318		Homo sapiens	breast		epithelial		female	0			fibrocystic disease	non-tumorigenic				none	adherent				"<a href = ""http://www.atcc.org/Products/All/CRL-10318"" target=""blank"">ATCC CRL-10318</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	51081-1	LCL-2066	SUM1315MO2	SUM1315M02, 1315M02, SUM1315, SUM 1315, SUM-1315, SUM 1315M02, SUM-1315M02, SUM1315-MO2	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009916""  target=""blank"">CLO_0009916</a>"	51081	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				University of Michigan (http://www.cancer.med.umich.edu/breast_cell/Production/index.html)														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51082-1	LCL-2067	SUM149PT	SUM 149, 149 PT, 149PT, SUM-149, SUM149, SUM-149PT, SUM 149PT	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009917""  target=""blank"">CLO_0009917</a>"	51082	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-149PT-p/cl%20sum-149pt.htm"" target=""blank"">Asterand SUM-149PT</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51083-1	LCL-2068	SUM159PT	159 PT, 159PT, SUM159, SUM-159PT, SUM 159PT, SUM-159, SUM 159	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009918""  target=""blank"">CLO_0009918</a>"	51083	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-159PT-p/cl%20sum-159pt.htm"" target=""blank"">Asterand SUM-159PT</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51084-1	LCL-2069	SUM185PE	SUM-185, SUM-185PE, SUM 185PE, SUM 185, SUM185, 185PE	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009919""  target=""blank"">CLO_0009919</a>"	51084	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-185PE-p/cl%20sum-185pe.htm"" target=""blank"">Asterand SUM-185PE</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51085-1	LCL-2070	SUM225CWN	225CWN, SUM-225CWN, SUM225, SUM 225, SUM-225, SUM 225CWN, SUM-225cwn, Sum225	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009920""  target=""blank"">CLO_0009920</a>"	51085	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-225CWN-p/cl%20sum-225cwn.htm"" target=""blank"">Asterand SUM-225CWN</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51086-1	LCL-2065	SUM229PE	SUM 229PE, SUM 229, SUM-229, 229PE, SUM229, SUM-229PE	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009921""  target=""blank"">CLO_0009921</a>"	51086	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-229PE-p/cl%20sum-229pe.htm"" target=""blank"">Asterand SUM-229PE</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51087-1	LCL-2071	SUM52PE	SUM-52-PE, SUM-52PE, SUM-52, SUM 52PE, 52PE, SUM52, SUM 52	"<a href = ""http://purl.obolibrary.org/obo/CLO_0037189""  target=""blank"">CLO_0037189</a>"	51087	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0								none	not specified				"<a href = ""http://solutions.asterand.com/SUM-52PE-p/cl%20sum-52pe.htm"" target=""blank"">Asterand SUM-52PE</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51088-1	LCL-2080	184A1	H184A1	"<a href = ""http://purl.obolibrary.org/obo/CLO_0001137""  target=""blank"">CLO_0001137</a>"	51088		HMS_LINCS	ATCC	CRL-8798		Homo sapiens	breast					0								none	adherent				"<a href = ""http://www.atcc.org/Products/All/CRL-8798"" target=""blank"">ATCC CRL-8798</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	51089-1	LCL-2082	MCF-12A	MCF12A, Michigan Cancer Foundation-12A	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007603""  target=""blank"">CLO_0007603</a>"	51089		HMS_LINCS	ATCC	CRL-10782		Homo sapiens	breast					0								none	adherent				"<a href = ""http://www.atcc.org/Products/All/CRL-10782"" target=""blank"">ATCC CRL-10782</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	51090-1	LCL-2072	MX-1	MX1, MXI	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009915""  target=""blank"">CLO_0009915</a>"	51090		HMS_LINCS	DTP, DCTD Tumor Repository (National Cancer Institute)			Homo sapiens	breast					0								none	not specified				"<a href = ""https://dtp.cancer.gov/repositories/default.htm"" target=""blank"">DTP, DCTD Tumor Repository, National Cancer Institute</a>"														Obtained by Joe Gray's group (Oregon Health & Science University) from the DTP, DCTD Tumor Repository		
20269	51091-1	LCL-2064	T47D-KBluc	T47DKBLUC, T47DKBluc	"<a href = ""http://purl.obolibrary.org/obo/CLO_0037191""  target=""blank"">CLO_0037191</a>"	51091		HMS_LINCS	ATCC	CRL-2865		Homo sapiens	breast					0								pGL2.TATA.Inr.luc.neo integration	adherent				"<a href = ""http://www.atcc.org/Products/All/CRL-2865"" target=""blank"">ATCC CRL-2865</a>"										The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. The cells were selected for responsiveness to 17-beta-estradiol.				Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC		
20269	51092-1	LCL-2073	600MPE	600 MPE, 600-MPE, MPE-600, MPE 600, MPE600, 600PE, 600-PEI	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009908""  target=""blank"">CLO_0009908</a>"	51092	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/3470538""  target=""blank"">PMID 3470538</a>"	HMS_LINCS	H.S. Smith (California Pacific Medical Center)			Homo sapiens	breast					0													H.S. Smith (California Pacific Medical Center)														Obtained by Joe Gray's group (Oregon Health & Science University) from H.S. Smith		
20269	51093-1	LCL-2074	HCC2185	HCC-2185, HCC 2185	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009909""  target=""blank"">CLO_0009909</a>"	51093	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/9833771""  target=""blank"">PMID 9833771</a>"	HMS_LINCS	Adi Gazdar (University of Texas-Southwestern Medical Center)			Homo sapiens	breast				female	38	white		DOID:3008, ductal carcinoma	breast ductal carcinoma				none	non-adherent				Adi Gazdar (University of Texas-Southwestern Medical Center)														Obtained by Joe Gray's group (Oregon Health & Science University) from Adi Gazdar		
20269	51094-1	LCL-2075	HCC3153	HCC 3153	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009910""  target=""blank"">CLO_0009910</a>"	51094		HMS_LINCS	Adi Gazdar (University of Texas-Southwestern Medical Center)			Homo sapiens	breast					0													Adi Gazdar (University of Texas-Southwestern Medical Center)														Obtained by Joe Gray's group (Oregon Health & Science University) from Adi Gazdar		
20269	51095-1	LCL-2076	MCF-7/LY2	LY2, LY-2	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009913""  target=""blank"">CLO_0009913</a>"	51095	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/4029083""  target=""blank"">PMID 4029083</a>"	HMS_LINCS	Georgetown University			Homo sapiens	breast					0								none					Mark Lippman (National Cancer Institute)										The cell line was selected by increasing the concentration of LY 117018, a potent antiestrogen that inhibits cell growth at concentrations as low as 10(-10) M, in the growth medium in a stepwise manner from 10(-8) to 10(-6) M as the cells become resistant.				Obtained by Joe Gray's group (Oregon Health & Science University) from an unknown researcher at Georgetown University		
20269	51096-1	LCL-2077	ZR-75-B	ZR75B, ZR-75B, ZR-75b, ZR75-B	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009924""  target=""blank"">CLO_0009924</a>"	51096	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/7439217""  target=""blank"">PMID 7439217</a>"	HMS_LINCS	Georgetown University			Homo sapiens	breast					0													Mark Lippman (National Cancer Institute)														Obtained by Joe Gray's group (Oregon Health & Science University) from an unknown researcher at Georgetown University		
20269	51107-1		21MT-1			51107	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/2487147""  target=""blank"">PMID 2487147</a>; <a href = ""http://www.ncbi.nlm.nih.gov/pubmed/1977518""  target=""blank"">PMID 1977518</a>"	HMS_LINCS	Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)			Homo sapiens	breast		epithelial		female	36			DOID:3005, invasive ductal carcinoma	breast ductal adenocarcinoma				none	adherent				Ruth Sager (Dana-Farber Cancer Institute)										established from the pleural effusion of lung metastases of a primary breast tumor				Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager		
20269	51108-1		21NT			51108	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/1977518""  target=""blank"">PMID 1977518</a>"	HMS_LINCS	Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)			Homo sapiens	breast		epithelial		female	36			DOID:3005, invasive ductal carcinoma	breast ductal adenocarcinoma				none	adherent				Ruth Sager (Dana-Farber Cancer Institute)										established from primary breast tumor				Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager		
20269	51109-1		21PT			51109	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/1977518""  target=""blank"">PMID 1977518</a>"	HMS_LINCS	Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)			Homo sapiens	breast		epithelial		female	36			DOID:3005, invasive ductal carcinoma	breast ductal adenocarcinoma				none	adherent				Ruth Sager (Dana-Farber Cancer Institute)										established from primary breast tumor				Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager		
20269	51110-1		CAL-120		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002179""  target=""blank"">CLO_0002179</a>"	51110		HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 549		Homo sapiens	breast				female	43			breast adenocarcinoma	DOID:3458				none	adherent; From DSMZ: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers	From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: split confluent culture 1:2 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 10<sup>6</sup> cells/175 cm<sup>2</sup>. Incubation: at 37C with 10% CO<sub>2</sub>. Doubling time: ca. 50-60 hours.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-459.html?tx_dsmzresources_pi5%5BreturnPid%5D=192"" target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 459</a>"										From DSMZ: established from the pleural effusion of a metastatic breast adenocarcinoma in 1991				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51111-1		CAL-148		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002181""  target=""blank"">CLO_0002181</a>"	51111	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/7531416""  target=""blank"">PMID 7531416</a>"	HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 460		Homo sapiens	breast		epithelioid		female	58			breast adenocarcinoma	DOID:3458				none	mixed; From DSMZ: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers	From DSMZ: Medium: 80% Dulbecco's MEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 g/100 ml). Subculture: Split confluent culture 1:4 to 1:10 every 3-5 days by agitation. Seed out at ca. 0.2-0.3 x 10<sup>6</sup> cells/ml (suspension culture) or 0.4-0.8 x 10<sup>5</sup> cells/cm<sup>2</sup> (adherent culture). Maintain at about 0.2-0.7 x 10<sup>6</sup> cells/ml or equivalent. Cell numbers may be difficult to ascertain due to growth in clumps. Cells grow faster in suspension without EGF. Incubation: at 37C with 10% CO<sub>2</sub>. Doubling time: ca. 30-40 hours.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-460.html?tx_dsmzresources_pi5%5BreturnPid%5D=192"" target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 460</a>"										From DSMZ: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol)				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51112-1		CAL-85-1		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002190""  target=""blank"">CLO_0002190</a>"	51112	Fischel, J. L., Formento, P., Lucas, C., Berlion, M., Bizzari, J. P., Milano, G. (1993) Le S9788 dans la rversion de la rsistance MDR: Activit compare  des composs de rfrence. <i>Bull Cancer</I> 80 : 409.	HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 440		Homo sapiens	breast		epithelial		female	35			breast adenocarcinoma	DOID:3458				none	adherent; From DSMZ: adherent epithelial-like cells growing in monolayers	From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate. Subculture: Split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (incubation with trypsin/EDTA for about 10 min). Seed out at ca. 0.03-0.04 x 10<sup>6</sup> cells/cm<sup>2</sup>. Maintain at 0.04 x 10<sup>6</sup> cells/cm<sup>2</sup>. A significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: ca. 70 hours.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-440.html?tx_dsmzresources_pi5%5BreturnPid%5D=192"" target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 440</a>"										From DSMZ: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51113-1		EFM-19		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002888""  target=""blank"">CLO_0002888</a>"	51113	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/6325325""  target=""blank"">PMID 6325325</a>"	HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 231		Homo sapiens	breast		epithelioid		female	50	Caucasian		breast ductal adenocarcinoma	DOID:3007				none	mixed; From DSMZ: epitheloid, growing in clusters at first, then as monolayer	From DSMZ: Medium: 90% RPMI 1640 + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA. Seed out at ca. 2 x 10<sup>6</sup> cells/25 cm<sup>2</sup>. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: about 64 hours.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-231.html?tx_dsmzresources_pi5%5BreturnPid%5D=192"" target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 231</a>"										From DSMZ: established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51114-1		EFM-192A		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002889""  target=""blank"">CLO_0002889</a>"	51114		HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 258		Homo sapiens	breast		epithelioid		female	46	Caucasian		breast carcinoma, breast adenocarcinoma	DOID:3459, DOID:3458	ERBB2 amplification	"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-258.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258</a>"		none	adherent; From DSMZ: epitheloid, adherent growing as monolayer	From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine. Subculture: Split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 2 x 10<sup>6</sup> cells/25 cm<sup>2</sup>. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: about 9 days.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-258.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258</a>"										From DSMZ: established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51115-1		EFM-192B		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002890""  target=""blank"">CLO_0002890</a>"	51115		HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 308		Homo sapiens	breast		epithelioid		female	46	Caucasian		breast adenocarcinoma, carcinoma	DOID:3458, http://purl.obolibrary.org/obo/DOID_305				none	adherent; From DSMZ: epitheloid adherent cells growing as monolayers	From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Subcultivation recommended before reaching confluence. Split 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 0.5-1.0 x 10<sup>5</sup> cells/cm<sup>2</sup>. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: ca. 4-5 days.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-308.html?tx_dsmzresources_pi5%5BreturnPid%5D=192"" target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 308</a>"										From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51116-1		EFM-192C		"<a href = ""http://purl.obolibrary.org/obo/CLO_0002891""  target=""blank"">CLO_0002891</a>"	51116		HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 311		Homo sapiens	breast		epithelioid		female	46	Caucasian		breast carcinoma, breast adenocarcinoma	DOID:3459, DOID:3458	ERBB2 amplification	"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-311.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311</a>"		none	adherent; From DSMZ: epitheloid adherent cells growing as monolayers	From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Optimal split ratio is 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 5-8 x 10<sup>4</sup> cells/cm<sup>2</sup> or 2 x 10<sup>6</sup> cells/25 cm<sup>2</sup> flask. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: ca. 4 days.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-311.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311</a>"										From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51117-1		HBL-100		"<a href = ""http://purl.obolibrary.org/obo/CLO_0003626""  target=""blank"">CLO_0003626</a>"	51117		HMS_LINCS	UCSF Cell Culture Facility			Homo sapiens	breast		epithelial		female	27	Caucasian		not diseased					none	adherent; From CLS: monolayer, adherent	From CLS: Medium: DMEM supplemented with 4.5 g/L glucose, L-glutamine, and 10% fetal bovine serum. Subculture: Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask); the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Split Ratio: A ratio of 1:2 is recommended. Fluid Renewal: 2 to 3 times weekly.		DNA Profile (STR, source: CLS): Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8 vWA: 16 D3S1358: 14,16 D21S11: 28,30 D18S51: 16 Penta E: 7 Penta D: 12 D8S1179: 12,15 FGA: 25	"<a href = ""http://www.clsgmbh.de/artikeldet.php?proid=775&sid=1lqkb92s17gr61jd5u6emkgph3"" target=""blank"">CLS Cell Lines Service 300178</a>"										From CLS: The epithelial cell line HBL-100 has been derived by E.V. Gaffney and associates from the milk of a nursing mother and obtained 3 days after delivery. Although there was no evidence of a breast lesion in the milk donor, and the patient had no family history of breast cancer, the karyotype of the recovered cells was abnormal as early as passage 7. This line was able to synthesize a small amount of lactose and would respond to prolactin or estrogen by producing increased amounts of casein. Electron micrographs revealed microvilli, tonofibrils and desmosomes.				Obtained by Joe Gray's group (Oregon Health & Science University) from UCSF CCF		
20269	51118-1		JIMT-1			51118	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/15634652""  target=""blank"">PMID 15634652</a>"	HMS_LINCS	Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures	ACC 589		Homo sapiens	breast		epithelial		female	62			breast carcinoma,  invasive ductal carcinoma	DOID:3459, DOID:3005	HER-2 amplification	"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-589.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589</a>"		none	adherent; From DSMZ: epithelial-like cells growing in monolayers	From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:8 every 2-4 days using trypsin/EDTA. Seed out at ca. 1 x 10<sup>6</sup> cells/80 cm<sup>2</sup>. Incubation: at 37C with 5% CO<sub>2</sub>. Doubling time: ca. 30-40 hours.			"<a href = ""https://www.dsmz.de/catalogues/details/culture/ACC-589.html""  target=""blank"">Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589</a>"										From DSMZ: established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)				Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ		
20269	51119-1		HMT-3522 S1			51119	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/3558253""  target=""blank"">PMID 3558253</a>"	HMS_LINCS	Mina Bissell (Lawrence Berkeley National Laboratory, University of California, Berkeley)			Homo sapiens	breast		epithelial		female	48	Caucasian		DOID:0060082, breast benign neoplasm		mutation at codon 179 of P53	"<a href = ""http://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=98102210&collection=ecacc_gc""  target=""blank"">European Collection of Authenticated Cell Cultures (ECACC) 98102210</a>"		none	adherent	From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 g/ml transferrin +10<sup>-8</sup> M sodium selenite + 10<sup>-10</sup> M 17 beta-estradiol + 0.5 g/ml hydrocortisone + 5 g/ml ovine prolactin + 10 ng/ml EGF			"<a href = ""https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=98102210&collection=ecacc_gc"" target=""blank"">European Collection of Authenticated Cell Cultures (ECACC) 98102210</a>"										From ECACC: HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. HMT-3522 S1 is a continuation of this line from passage 34 growing without collagen.				Obtained by Joe Gray's group (Oregon Health & Science University) from Mina Bissell		
20269	51120-1		SUM102PT			51120	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0			DOID:3005, invasive ductal carcinoma	breast ductal adenocarcinoma				none	adherent	From Asterand: Hams F-12 + 1 g/L bovine serum albumin (fatty acid-free), 10 ng/mL EGF, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine			"<a href = ""http://solutions.asterand.com/SUM-102PT-p/cl-sum-102pt.htm"" target=""blank"">Asterand SUM-102PT</a>"										From Asterand: developed from a patient with minimally invasive, ER negative and PR negative human breast carcinoma.				Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51121-1		SUM190PT			51121	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0			DOID:6263, inflammatory breast carcinoma	inflammatory luminal breast carcinoma				none	adherent	From Asterand: Hams F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine			"<a href = ""http://solutions.asterand.com/SUM-190PT-p/cl%20sum-190pt.htm"" target=""blank"">Asterand SUM-190PT</a>"										From Asterand: derived from a primary human inflammatory luminal breast carcinoma				Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51122-1		SUM44PE			51122	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/10604729""  target=""blank"">PMID 10604729</a>"	HMS_LINCS	Stephen Ethier (Wayne State University and University of Michigan Medical School)			Homo sapiens	breast					0			DOID:3459, breast carcinoma	luminal breast carcinoma				none	adherent	From Asterand: Hams F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine			"<a href = ""http://solutions.asterand.com/SUM-44PE-p/cl%20sum-44pe.htm"" target=""blank"">Asterand SUM-44PE</a>"										From Asterand: derived from primary human luminal breast epithelial cells of a pleural effusion.				Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier		
20269	51123-1		HMT-3522 T4-2			51123	"<a href = ""http://www.ncbi.nlm.nih.gov/pubmed/3558253""  target=""blank"">PMID 3558253</a>"	HMS_LINCS	Mina Bissell (Lawrence Berkeley National Laboratory, University of California, Berkeley)			Homo sapiens	breast		epithelial		female	48	Caucasian		DOID:0060082, breast benign neoplasm		mutation at codon 179 of P53; trisomy for the short arm of chromosome 7	"<a href = ""https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=98102212&collection=ecacc_gc"" target=""blank"">European Collection of Authenticated Cell Cultures (ECACC) 98102212</a>"		none	adherent	From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 g / ml transferrin +10<sup>-8</sup> M Sodium selenite + 10<sup>-10</sup> M 17 beta-estradiol + 0.5 g/ml hydrocortisone + 5 g / ml ovine prolactin		DNA Profile (STR, source: Asterand): STR-PCR Data: Amelogenin: X CSF1PO: 11,12  D13S317: 11 D16S539: 11,13  D5S818: 9,11 D7S 820: 8,9 THO1: 9,9.3  TPOX: 8,11 vWA: 14,17	"<a href = ""https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=98102212&collection=ecacc_gc"" target=""blank"">European Collection of Authenticated Cell Cultures (ECACC) 98102212</a>"										From ECACC: HMT-3522 T4-2, also known as HMT-3522/mt-1, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line at passage 25-30. The subline S1 is a continuation of this line from passage 34 (ECACC cataogue no. 98102210) growing without collagen. Subline S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). The third subline, T4-2 was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines.				Obtained by Joe Gray's group (Oregon Health & Science University) from Mina Bissell		
