centerdatasetid	cl_center_batch_id	cl_lincs_id	cl_name	cl_alternative_names	cl_alternative_ids	cl_center_canonical_id	cl_relevant_citations	cl_center_name	cl_provider_name	cl_provider_catalog_id	cl_provider_batch_id	cl_organism	cl_organ	cl_tissue	cl_cell_type	cl_cell_type_detail	cl_donor_sex	cl_donor_age	cl_donor_ethnicity	cl_donor_health_status	cl_disease	cl_disease_detail	cl_known_mutations	cl_mutation_citations	cl_molecular_features	cl_genetic_modification	cl_growth_properties	cl_recomended_culture_conditions	cl_related_projects	cl_verification_reference_profile	cl_reference_source	cl_cell_markers	cl_gonosome_code	cl_disease_site_onset	cl_disease_age_onset	cl_donor_age_death	cl_donor_disease_duration	cl_precursor_cell_name	cl_precursor_cell_lincs_id	cl_precursor_cell_center_batch_id	cl_production_details	cl_quality_verification	cl_transient_modification	cl_passage_number	cl_source_information	cl_date_received	cl_center_specific_code
20237	50029-3	LCL-1460	MCF-7	ssMCF-7, MCF 7, Michigan Cancer Foundation-7, MCF7/WT, MCF7-CTRL, IBMF-7, ssMCF7, MCF.7, MCF7	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007606""  target=""blank"">CLO_0007606</a>"	50029		HMS_LINCS	ATCC	HTB-22		Homo sapiens	breast		epithelial		female	0	Caucasian	disease	breast adenocarcinoma	DOID:3458		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905946""  target=""blank"">COSS905946</a>"		none	adherent	From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%. Protocol: Remove culture medium to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended		(1.)DNA Profile (STR, source: ATCC): Amelogenin: X  CSF1PO: 10  D13S317: 11  D16S539: 11,12  D5S818: 11,12  D7S820: 8,9  THO1: 6  TPOX: 9,12  vWA: 14,15 (2.)STR_Amelogenin_X, STR_CSF1PO_10, STR_D13S317_11, STR_D16S539_11_12, STR_D5S818_11_12, STR_D7S820_8_9, STR_THO1_6, STR_TPOX_9_12, STR_vWA_14_15	"<a href = ""http://www.atcc.org/Products/All/HTB-22"" target=""blank"">ATCC HTB-22</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010.	2010-07-16	
20237	50057-3	LCL-1475	SK-BR-3	Sk-Br-3, SKBR3, SkBr3, SK-Br-3, SKBr3, SKBr-3, SK-BR3, SKBR-3	"<a href = ""http://purl.obolibrary.org/obo/CLO_0009034""  target=""blank"">CLO_0009034</a>"	50057		HMS_LINCS	ATCC	HTB-30		Homo sapiens	breast	breast	epithelial		female	0	Caucasian		adenocarcinoma, breast carcinoma	DOID:3459				none	adherent	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5%  Temperature: 37.0 deg C  Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended  Medium Renewal: 2 to 3 times per week		DNA Profile (STR, source: ATCC):  Amelogenin: X  CSF1PO: 12  D13S317: 11,12  D16S539: 9  D5S818: 9,12  D7S820: 9,12  THO1: 8,9  TPOX: 8,11  vWA: 17	"<a href = ""http://www.atcc.org/Products/All/HTB-30"" target=""blank"">ATCC HTB-30</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010	2010-07-16	
20237	50058-4	LCL-1461	MDA-MB-231	MDA Mb231, MDA MB231, MDA-MB 231, MDA.MB.231, MDA MB 231, MB231, MDA231, MDA-231, MDAMB231, MDA-MB231	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007634""  target=""blank"">CLO_0007634</a>"	50058		HMS_LINCS	ATCC	HTB-26		Homo sapiens	breast	breast	epithelial		female	0	Caucasian		breast adenocarcinoma	DOID:3458		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905960""  target=""blank"">COSS905960</a>"		none	adherent	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100%  Temperature: 37.0 deg C  Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended  Medium Renewal: 2 to 3 times per week		DNA Profile (STR, source: ATCC):  Amelogenin: X  CSF1PO: 12,13  D13S317: 13  D16S539: 12  D5S818: 12  D7S820: 8,9  THO1: 7,9.3  TPOX: 8,9  vWA: 15,18	"<a href = ""http://www.atcc.org/Products/All/HTB-26"" target=""blank"">ATCC HTB-26</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010	2010-07-16	
20237	50105-3	LCL-1476	BT-20	BT20, BT 20	"<a href = ""http://purl.obolibrary.org/obo/CLO_0002041""  target=""blank"">CLO_0002041</a>"	50105		HMS_LINCS	ATCC	HTB-19		Homo sapiens	breast	breast			female	0	Caucasian		ductal carcinoma, breast carcinoma	DOID:3459		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=906801""  target=""blank"">COSS906801</a>"		none	adherent	From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-19"" target=""blank"">ATCC HTB-19</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010	2010-07-16	
20237	50238-3	LCL-1315	Hs 578T	Hs-578-T, HS 578T, Hs-578T, HS-578T, Hs_578t, HS-578-T, Hs 578.T, HS578T, Hs578T, Hs578t, HS0578T, 578T, HS578, Hs578, Homo sapiens No. 578, tumor cells	"<a href = ""http://purl.obolibrary.org/obo/CLO_0004009""  target=""blank"">CLO_0004009</a>"	50238		HMS_LINCS	ATCC	HTB-126		Homo sapiens	breast	breast			female	0	Caucasian		breast ductal carcinoma	DOID:3008		"<a href = ""http://cancer.sanger.ac.uk/cell_lines/sample/overview?id=905957""  target=""blank"">COSS905957</a>"		none	adherent	From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mg/ml bovine insulin and 10% fetal bovine serum. Protocol:  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended			"<a href = ""http://www.atcc.org/Products/All/HTB-126"" target=""blank"">ATCC HTB-126</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010	2010-07-16	
20237	50583-7	LCL-2085	MCF-10A	Michigan Cancer Foundation-10A, MCF10a, MCF10A, MCF.10A, MCF 10A	"<a href = ""http://purl.obolibrary.org/obo/CLO_0007599""  target=""blank"">CLO_0007599</a>"	50583		HMS_LINCS	ATCC	CRL-10317		Homo sapiens	breast	breast, mammary_gland_connective_tissue	epithelial		female	0	Caucasian	disease	breast fibrocystic disease, fibrocystic disease	non-tumorigenic				none	adherent			Isoenzymes#_AK1#_1, Isoenzymes#_ESD#_1, Isoenzymes#_G6PD#_B, Isoenzymes#_GLO1#_1#_2, Isoenzymes#_PGM1#_1#_2, Isoenzymes#_PGM3#_1, STR#_Amelogenin#_X, STR#_CSF1PO#_10#_12, STR#_D13S317#_8#_9, STR#_D16S539#_11#_12, STR#_D5S818#_10#_13, STR#_D7S820#_10#_11, STR#_THO1#_8#_93, STR#_TPOX#_9#_11, STR#_vWA#_15#_17	"<a href = ""http://www.atcc.org/Products/All/CRL-10317"" target=""blank"">ATCC CRL-10317</a>"											PASS			Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010	2010-07-16	
